Madonna was just the closing comic in a powerhouse lineup of all-stars, and the “Material Girl” killed.

It all went down at the famous Comedy Cellar in NYC.

We’re told the scheduled lineup of comics got bumped because a murderers’ row showed up, including Sean Donnelly, Pete Holmes, Aziz Ansari, Louis C.K., Jon Stewart, and Amy Schumer.

Amy Schumer, who opened for Madonna’s concerts, brought the singer on stage and turned her loose on the crowd.

We’re told Madonna had everyone doubling over during a particularly raunchy set of jokes centered around her … vagina.  She was the closing set and absolutely destroyed the audience.

Afterwards, Madonna and the rest of the comics all hung out and mingled.

Imagine getting to see Madonna on stage for the price of a two drink minimum.

Read more at The Blast